The sale will see the motorcycle raise funds for Caritas Roma, which works on behalf of the Roman Catholic church to support those in need around the world.
Pope Francis donated the bike to help raise funds for the charity. It carries an estimate of €12,000-15,000.
Signed on the tank by his Holiness at a special ceremony at the Vatican in November, the machine is also offered with a certificate of conformity from Harley-Davidson.
Ben Walker, head of motorcycles at Bonhams, said: “We are incredibly honoured to be selling this item on behalf of Caritas Roma. We hope to be able to do both Pope Francis and Harley-Davidson proud by raising a significant amount of money for a very worthy cause.”
The Harley-Davidson will be sold on 6 February 2014 as part of Bonhams’ Les Grandes Marques du Monde sale at the Grand Palais in Paris.